In February 2023, the EDQM and European Partnership for Alternative Approaches to Animal Testing (EPAA) hosted a landmark conference to present the European Pharmacopoeia (Ph. Eur.) Commission’s strategy to phase out the rabbit pyrogen test (RPT) and transition to modern, animal-free alternatives. This shift was guided by ethical imperatives in line with the principles of Directive 2010/63/EU on the protection of animals used for scientific purposes, and grounded in state-of-the-art scientific innovation, with a strong focus on enhancing patient safety.